This isn't the first time the media mogul has thrown shade at one of King's looks.
It's hard to imagine Oprah Winfrey hating many things in life, but unfortunately for Gayle King, her necklace is one of them.
The Big O was not a fan of the bold accessory her BFF wore on Tuesday's CBS This Morning, and according to King, sent a message to her assistant complaining about the fashion choice.
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"Note @oprah sent to my asst this am 'plz tell Gayle I'm on treadmill & it's hard to focus w/the circus around your neck,'" King wrote alongside an image of the necklace in question. "I think she's WRONG thoughts?"
While it's hard to imagine any friendship surviving that kind of shade, it's something the famous friends have been through before.
King also took to Instagram last year to vent about Winfrey's critique of her lip color.
"@Oprah just called & said tell your makeup artist waaaay too red on the lips today," she captioned a snap of her on-camera look. "I said proudly 'I did that MYSELF' Dead silence on the line ... Hello ??hello?..."
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While Oprah disagrees with a few of her BFF's fashion and beauty choices, she still looked to King for help when creating this year's list of her famous favorite things.
Only ET caught up with King to get the scoop on this season's Oprah-approved gifts (SPOILER: her necklace did not make the list); see her favorites in the video below.