The cancellation comes just days after the actor dropped out of 'Six', also due to health.
Joe Manganiello pulled out of a charity event he was supposed to co-chair with his wife, Sofia Vergara, on Sunday, apparently due to health issues.
The couple was scheduled to act as honorary co-chairs at the Stuart House Benefit in West Hollywood, California, to raise funds for the facility, which helps children and families recover from sexual abuse.
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A representative at the event told ET's Lauren Zima that the Magic Mike XXL star dropped out due to "health issues."
Fashion designer John Varvatos, who hosts the event every year and is a long-time friend of the couple, said he had extended the invitation to co-chair this year's event in recognition of Manganiello's lengthy support of Stuart House.
"Joe's been a big part of what we've done over the years. He's been a great personal friend. He's been involved in all of our – the last four or five years – our Stuart House events," Varvatos told ET.
"Basically they just couldn't make it because [Manganiello] is recovering," he added. "I don't know a lot about it other than that he's had a little bit of a health problem. We wish him the best. We thank him for all his support that he's given us and, most importantly, to get well soon because he's such a wonderful guy."
Manganiello hasn't addressed the issues directly in public, but he did post a simple tweet on Sunday afternoon: "I love my wife."
This isn't the first health-related incident recently for the 39-year-old actor. Just last week it was announced the True Blood alum would be bowing out of History Channel's Navy SEAL drama series Six reportedly for health reasons and the physical demands of the series.
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ET has reached out to the couple's reps for more information.
The beefy heartthrob recently got to stretch his comedic chops in Tumbledown opposite Rebecca Hall and Jason Sudeikis. Check out more in the clip below.