The Women of Charlie Sheen's Life Speak Out

After Charlie Sheen confirmed he is HIV positive in a Today show appearance on Tuesday, many of the women in his past have spoken out about Sheen's health and what this means for them -- and the public's perception.

Sheen's primary physician, Dr. Robert Huizenga explained to ET that the actor has had "consistently undetectable" levels of HIV in his blood due to effective medicine, but the news was still difficult for his loved ones to hear.

WATCH: Charlie Sheen Reveals He's HIV Positive

The 50-year-old Golden Globe winner also told Today that he only recently informed his oldest daughter, 30-year-old Cassandra Estevez, of his diagnosis. "I felt bad, it hit her hard," Sheen admitted. "But she recovered, and she's tough like her dad."

Sheen's ex-wives, Denise Richards and Brooke Mueller, as well as his children with them are all HIV negative. While the actor said that he told Richards and Mueller about his HIV status in 2011, ex-girlfriend Bree Olson, who dated the actor in 2011, claims that she was never notified. "He's a monster," she recently told Inside Edition.

Despite Olson's anger, many of the women in Sheen's life offered messages of support. 

MORE: Could Charlie Sheen Face Legal Action After Revealing He Is HIV Positive?

Read on for statements from the women who knew Sheen intimately.

Brooke Mueller, Sheen's third ex-wife:

Mueller, who was married to the former Two and a Half Men star from 2008-11, confirmed to ET through her rep Steve Honig that she and her 6-year-old twin sons Bob and Max are not HIV positive following Sheen's revealing Today show interview.

"Brooke has been inundated with calls from friends and family," the statement read. "To put their minds at ease, Brooke can confirm that she and the boys are not HIV positive."

Denise Richards, Sheen's second ex-wife:

Richards split from Sheen in 2006 after four years of marriage, and shared what Sheen was like at the beginning of their relationship on Allegedly with Theo Von two weeks prior to Sheen's Today show announcement.

"Charlie, when I married him, he wasn't a bad boy," Richards said. "He was sober for almost four years. He was a very different person. His lifestyle was very, very different. He was a former bad boy, I guess."

Mia Isabella, Sheen's ex-girlfriend:

The transgender model and adult film star was linked to Sheen in 2014. She gave an exclusive statement to ET concerning Sheen's announcement.

"Prayers out to him," she said. "That IS hard news to hear. I'm saddened but know him as an incredible person and advocate. My memories of him reflect a warrior and super brave person, so I know he will navigate through this with strength. He isn't afraid of facing any public scrutiny and speaking frankly and I have nothing but admiration for his friendship.

"He was a great mentor to me during the time he was a part of my life years ago. I was very fortunate to have his support when media had once threatened to out our friendship and relationship and he shielded me from public scrutiny. Me being faced with losing my privacy lead to us going our separate ways but I truly hope this is seen as a teachable moment to educate versus public humiliation and shame. This is a serious situation and it's important for people to know it could happen to anyone and affect anyone's life no matter who they are. It's clearly a violation of his privacy yet a lesson in safe sex, testing, being proactive with your health and knowing your status. I have yet to speak to him but my prayers go out to him and his family during this time."

Natalie Kenly, Sheen's ex-girlfriend:

Kenly lived in Sheen's L.A. mansion in 2011 as part of his posse of girlfriends that he nicknamed his "goddesses." She gave the following statement to People.

"He is not a monster. He has regard for human beings and cares about people. I do not see him knowingly putting women at risk.

"That's not who he is. He's got young kids. He's also not an idiot and I don't see him opening himself up to that legal nightmare.

"He's a good man and I feel very fortunate to have spent the time with him that I did, and I'm so sad to hear of his health troubles. I hope he's okay."

Bree Olson, Sheen's ex-girlfriend:

The former adult film star was also a part of Sheen's "goddess" crew in 2011, and she addressed Sheen's HIV reports via Twitter.

"There are speculations circling that one of my ex boyfriends from years ago may have contracted the HIV virus," she wrote. "They are rumors and I know nothing more than anyone else.

"I, myself -- have been to the gynecologist once a year since we split up and have always been tested across the board for everything and have come back clean across the board every time. For all the 'news' sources out there, leave me alone. Call me Jon Snow. I know nothing."

She returned to Twitter on Monday, writing, "Recorded myself for half an hour being tested for HIV and getting the results. This is so stressful."

Heather Locklear, Sheen's former Spin City co-star:

The actress, who worked with Sheen for six years on the ABC sitcom, took to Instagram on Monday, posting a throwback picture of the two of them together along with a message.

"My heart hurts," she wrote. "Prayers for Charlie and his family."

Heidi Fleiss, Sheen's friend and former madame:

Fleiss hasn't talked to Sheen since before the 1995 trial in which he admitted to spending $53,000 on 11 of her prostitutes, but she sat down with ET on Tuesday following Sheen's announcement, emphasizing that there were no hard feelings between them.

"I think Charlie could -- it's a very important role he has -- that he change the stigma attached that people who have it [HIV] feel," Fleiss told ET. "I think a lot more people have it than we realize. But it's like asthma -- big deal -- so you take some medication. It's not a death sentence or something, and it shouldn't be this dirty little secret. Some of the people -- I've seen how they underachieve, how they drink and they act out -- and it's sad because it's so unnecessary."

Amanda Bruce, Sheen's ex-girlfriend:

Bruce, a nurse, told Dr. Oz that she had unprotected sex with Charlie after he told her he was HIV positive.

"I mean, if I had met him on one night, and we had decided to engage in this, I'd say, 'That's Russian Roulette,'" she explained. "The reality is we had known each other for many months before we engaged in [sex], and we were a couple. I was in love with him."